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This edition contains procedures for the new DR/2500 Spectrophotometer, electrochemical procedures for the sensION family of products, and titrimetric procedures for Hach's. DR 2800 2495402 Fill line faces right. Process Grain Shabbat Search Engine JewJewJew.com you could look here.
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Chloride and sulfate were determined with the HACH DR 2500 spectrophotometer, free chlorine was measured with HACH DR 890. The UVS uses an ultraviolet diffuser to obtain large angular response with no aiming. The concentration levels of sulphate were analysed using the HACH Spectrophotometer model No DR/2400 whereas those of Cl, SO (1.60 to 3.55 mg/l) for the rainwater and HCO 3 – (73.30 to 273.
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I then punch out the cell(s) of interest using a hole punch and attach the 5mm circles to blank stubs using quick setting epoxi-patch bond (hardens in 5-10 minutes). AgroGene seeds and Crop genetics pvt Limited was established in 2020 by Experienced Agricultural professional to meet food deficit by providing Hi yielding and Disease resistant quality Hybrids seeds and to develop farming community in the Indian Seed Industry. Total hardness and total alkalinity were determined using standard titrimetric methods5-6.
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Colorimeter A colorimeter / spectrophotometer capable of measuring absorbance at 600nm was used. Various liquid chromatography (LC) methods are promising tools for relatively fast and exact quantification of lipopeptides. Hach Sample Cell at Thomas Scientific.
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Hach services DR/2020 Spectrophotometers (Catalog No. 4480000) that have software version 3.0 or greater. It's a microprocessor-controlled single beam instrument suitable for the laboratory or the field. This is available from Dexter Corporation 1-603-474-5545 and works extremely well.
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Penn Center Boulevard, Suite 332 Pittsburgh, PA 15235-5503 USA Stay up-to-date on all Pittcon news. Forbes stated that he also has a Request to Purchase for MS4 from HACH Company. Ammonia-N, iron, residual chlorine and total chlorine were determined by spectrophotometer according to the standard procedures4.
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Standard analysis with 130+ pre-programmed methods; Not compatible with TNTplus; Works with all other Hach chemistries; Runs on Either Line Power or Battery Power; Use a USB Memory Stick to Update the Instrument or Transfer Data Hazardous Items. Collection of samples and analytical procedures. The MS4 are materials (a spectrophotometer and ammonia) which are used to track the outfalls in the ditches throughout town.
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Add the same iron concentration to the ammonia-free water in Step 4. The interference will then be successfully blanked out. The DR3900 is a benchtop visible spectrum (320 - 1100 nm), split beam spectrophotometer with over 220 pre-programmed methods optimized for laboratory water analysis. Item(s) are sold-as-is as shown in pictures above.
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The sipper system is powered via USB connection to the DR 3900 and is controlled through the DR 3900 menu. Although many biochemicals are colored, as in, they absorb visible light and therefore can be measured by colorimetric procedures, even colorless biochemicals can often be converted to colored compounds suitable for chromogenic. Analytical Instruments - Pcr Thermal Cycler you could check here.
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[A LEVELS] H2 Biology Practical FINAL TIPS
To everyone taking the H2 Bio prac on Thursday all the best!!! Here's some points that you should know by the end of tomorrow to do well and I'm just listing them out as a self-reminder as well :)
- Size (at least 2/3 of given space)
- Proportion (correct ratio of dimensions of each part)
- Line (sharp and continuous; erase all stray marks!)
- Revise the basic components of a plant cell and plant tissues (diffierentiate a plan & cell diagram!)
- Root (Epidermis, cortex, xylem, phloem, cambium, vascular bundle)
- Stem (Epidermis, cortex, pith, xylem, phloem, cambium, vascular budle)
- Leaf (Uppder epidermis, waxy cuticle, palisade mesophyll, spongy mesophyll, xylem, phloem, vascular bundle, guard cell, stoma, lower epidermis)
- No awkward scales
- Title (if required by question)
- Correct axes with units
- Check question for LINE or CURVE of best fit
- DO NOT EXTRAPOLATE (even if its tempting to connect the graph to origin when you shouldn't)
- Read and record to 1/2 of smallest division of instrument (unless instructed otherwise; e.g. "if the colour does not change after 10 minutes, record 'no observable change' " <--- follow this strictly!)
- Average values follow d.p. of raw data
- Processed data take on s.f. of least precise raw data
|Objective lens||Actual magnification||Number of stage micrometer divisions||Number of divisions on eyepiece graticule that this coincides with||Length of each eyepiece graticule division|
Study the common questions tested that require content knowledge from the H2 syllabus. Here is a list of some of them.
- Rate of enzyme activity when change in factors, including enzyme conc, substrate conc, temperature, pH, presence of competitive / non-competitive / allosteric inhibitors
- Keywords : Frequency of effective collision, rate of enzyme-substrate complex formation, active site, complementary in shape and charge, denaturation, disruption of R-group interactions that stabilised 3D tertiary conformation of enzyme active site
- Rate of photosynthesis when change in factors, including light intensity, CO2 conc, temperature
- Keywords : Photoactivation, resonance transfer of energy, electron hole, photolysis resulting in production of O2, carbon fixation
- Rate of respiration when change in factors, including temperature, glucose conc, O2 conc
- Keywords : Oxidative phosphorylation, H+ gradient across inner mitochondrial membrane, proton-motive force, chemiosmosis, O2 as final electron acceptor
- Biomolecules + test for presence (may be asked to describe)
Q: Possible limitations of the procedure
A: Inaccurate and subjective judgement of colour change by the human eye (if identifying colour change is involved like Benedict's test or DCPIP) / Small sample size resulting in high percentage error / Lack of control / Lack of repeats and replicates
Q: Count the number of cells in the microscope field of view undre x40 objective lens.
A: Record 3 counts and take average!
Q: Purpose of conducting a pilot test
A: To be familiair with when end-point is reached so this can be used as a standard for subsequent tests
Q: Simple dilution VS serial dilution
A: Simple dilution is more accurate since error in early dilutions will not be carried forwards.
Aim - Copy from question; Otherwise "To investigate how [independent variable] affects [dependent variable]
Theory - People tend to over-write but this section is only worth 1-2 marks and there are usually many possible mark points so don't waste too much time on this~~
Variables - Independent + Range; Dependent; Measurable + How to convert from measurable to dependent; Constant + Why constant + How to keep constant
Procedures - Pilot test, annotated diagram (if the set-up is rather complicated), control, replicates and repeats
Data processing - Table with appropriate headings (REMEMBER to record "replicate 1", "replicate 2", "replicate 3" and "average") + Graph if necessary + How to address aim with the collected data
Risks and precautions - Identify potential danger + How you prevent them
*Common risks and precautions
- Hot water, so use rags to hold container to prevent scalding (when water bath is needed)
- Sharp items, so place them on non-slip surfaces to prevent accidents (when cutting of plant / animal specimens is required)
- Reagent may be an irritant / allergen (literally can be said for ANY reagent except distilled water, like enzyme solutions, H2O2, DCPIP etc.), so wear safety goggles and gloves to prevent contact with eyes and skin
- Use of ethanol / alcohol (for dissolving of cell membranes), so ensure no naked flame nearby to prevent catching fire
- Use of electrical applicances (e.g. colorimeter, spectrophotometer, bench lamp, centrifuge, blender etc.), so operate with dry hands to prevent electrocution
- Max capacity of spectrophotometer cuvette ==> 1cm3; use a micropipette
- Max capacity of petri dish ==> 90cm3, but keep to 50cm3
e.g. Use a clean 5.0cm3 syringe, transfer 3.0cm3 of protease solution into a boiling tube.
That's it for now! Do suggest below for other important things to note and any predictions for the paper HAHAHAHA
Items to bring:
Photo ID / Passport
Compass (just in case you need to draw round cells and you can't draw them freehand like me)
Notes to study (if your shift allows you to)
Snacks (if your shift allows you to)
Sleep early, rest well and all the best!!!
Edit: Updated microscopy table